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IQ Chamber Test Instructions



IQ Chamber test instructions


The IQ chambers are supplied in units of 10 chambers on a hypoallergenic non woven adhesive tape. The tape with the chambers is protected by a stiff plastic cover with 10 compartments that correspond to the chambers on the tape.






Patch Test Technique


A 1. Put the IQ unit on a table, or use the Chemotechnique application device. Take a firm grip on either the right or the left perforated corner of the plastic cover.

2. Pull off the perforated corner of the plastic cover by bending it downwards over the edge of the cover so that the perforation will brake.

3. Pull back the tape gently until all chambers are available. The tape should not be loosened from the cover.

4. Apply the test preparation into the upper right chamber. Proceed with the other chambers until all are filled and the test unit is ready for application onto the patients back. Remember that when applied to the back, the order of the haptens will be left to right reversed, that is, the upper right chamber will be no.1 at left.

5. Grip the end of the tape and the plastic cover (picture A5). Press the tape to the back from below upwards. Remove the plastic cover (picture A6). For maximum adhesion press the palm of the hand to the tape for about 5 seconds. Using IQ Chambers, a big advantage is that you can prepare several test units in advance and store them in your refrigerator in a closed plastic bag up to 1-2 weeks. If you wish to adopt this method, put the tape back onto the plastic cover and mark the unit with the name and numbers of the test series (e.g. Standard 1-10).


B. When applying liquid haptens, apply a drop of the test solution to the filter paper in the chamber (about 25 μl). The amount should be just enough to properly moisten the filter paper. Press the filter paper down into the chamber with a pair of tweezers. Do not prepare liquid solutions in advance for storage.

C. When applying the test patches on the patient, use if possible the upper part of the patients back, and avoid to apply patches to the midline and the scapula.


In some cases other areas of the body like the upper part of the arms may be used. If the patients skin is oily you can clean it gently with some ethanol. Instruct the patient to bend gently forward when you apply the test units.

D. Mark to the left of the tape the first and the 5th chamber with the special Chemotechnique Skin Marker. Only for special difficult cases you will need to apply extra reinforcement tape. Make a record on a record form of the numbers and names of each hapten starting with no. 1 of the left upper patch and going

down and continue then to the right until all patches are recorded. The most convenient way of making such record forms is to use the Chemotechnique web site at

E. The patches should be removed after 48 hours. Reading of the test is performed 72 hours after the test application. In many instances, a second reading a week after the application is of value. To facilitate the reading, the special IQ Chambers Reading Plate could be used. During the time the patches remain on the back the patient should not shower, perform hard work or do exercise that will result in sweating. The back should not be rubbed by e.g. a brassiere or be scratched in the case of positive reactions. The patient should not take any cortisone medication during the test and avoid exposure of the back to the sun.

For the interpretation of the test result the following scheme can be used;

- Negative reaction

? Doubtful reaction

+ Weak reaction (non-vesicular)

++ Strong reaction (oedematous or vesicular)

+++ Extreme reaction (ulcerative or bullous)

IR Irritant reaction

NT Not tested


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